猪SERPINC1基因对PCV2复制的效应及其转录调控

doi:10.11843/j.issn.0366-6964.2019.08.003

Abstract

Abstract: The aim of this study was to investigate the effect of porcine SERPINC1 gene on PCV2 replication and to explore the transcriptional regulation of SERPINC1 gene. In this study, 15 purebred Laiwu pigs (LW) and 15 commercial Yorkshire×Landrace hybrid pigs (YL) were used. There were 2 groups in each breed, one for the challenged group (10) and one for the control group (5). Pigs in the challenged group were intramuscularly injected with 3 mL of 6.3×10^-3TCID₅₀ PCV2-SD strain, and pigs in control group were intramuscularly injected with 3 mL of phosphate buffer. Based on the previous transcriptome sequencing, the effect of SERPINC1 gene on PCV2 replication was analyzed, and the promoter and transcriptional activity of SERPINC1 gene were detected. The results indicated that over-expression of SERPINC1 could significantly inhibit the replication of PCV2 in PAM cells. The 3 854 bp sequence of the 5' regulatory region of the SERPINC1 gene in LW and YL pigs was cloned respectively, and the promoter activity was analyzed. It was found that the promoter activity of SERPINC1 gene in LW pigs significantly increased after PCV2 infection(P<0.05), while that of YL pig didn't significantly change. Four critical regulatory regions were found in the 5' regulatory region of the SERPINC1 gene. Four polymorphic sites were identified in the critical regulatory regions in LW and YL pigs. By site-directed mutation and dual luciferase reporter gene assay, we found that the activity of the SERPINC1 promoter was not affected by any one of the polymorphic sites in the above regions. The results laid a foundation for finding the gene and molecular markers related to the resistance of PCV2.

CLC Number:

S828.2

WANG Changying, LU Hongyu, SUI Minmin, WANG Yanchao, LIU Gen, SUN Yi, JIANG Yunliang. Effect of SERPINC1 Gene on PCV2 Replication and Its Transcriptional Regulation[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1545-1553.

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Effect of SERPINC1 Gene on PCV2 Replication and Its Transcriptional Regulation

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